| 质粒名称: | pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 61629 |
| 存储实验室: | Dolf Weijers |
|
载体骨架基本信息 |
|
| 载体名称: | pGreenII |
| 载体出品公司: | -- |
| 载体抗性: | Kanamycin low (25 ug/ml) |
| 空载体大小: | 2580 bp |
| 完整质粒大小: | 12791 bp |
| 载体修饰: | -- |
| 载体类型: | 植物表达 |
| 筛选标记: | Methothrexate |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | mDII,Fragment of Arabidopsis IAA28, mutated to prevent auxin-dependent degradation |
| 物种来源: | A. thaliana (mustard weed) |
| 插入大小: | 102 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 |
RPS5A
|
| 标签/融合蛋白: | -- |
| 克隆方法: | Ligation Independent克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | M13_forward20_primer |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus was a gift from Dolf Weijers (Addgene plasmid # 61629 ; http://n2t.net/addgene:61629 ; RRID:Addgene_61629) |
| 参考文献部分: | Reporters for sensitive and quantitative measurement of auxin response. Liao CY, Smet W, Brunoud G, Yoshida S, Vernoux T, Weijers D. Nat Methods. 2015 Mar;12(3):207-10. doi: 10.1038/nmeth.3279. Epub 2015 Feb 2. 10.1038/nmeth.3279 PubMed 25643149 |
买家导航
pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus载体质粒基本信息
pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus质粒图谱载体图谱和pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus载体序列质粒序列多克隆位点信息
pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus质粒载体简介
pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus质粒序列载体序列
LOCUS pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus 12920 bp ds-DNA circular SYN 27-FEB-2019
DEFINITION R2D2: Expresses a fusion of auxin-sensitive DII to nuclear 3xVenus
and auxin-insensitive mDII to nuclear tdTomato, both from the
Arabidopsis RPS5A promoter.
ACCESSION .
VERSION .
KEYWORDS pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12920)
AUTHORS Liao CY, Smet W, Brunoud G, Yoshida S, Vernoux T, Weijers D
TITLE Reporters for sensitive and quantitative measurement of auxin
response.
JOURNAL Nat Methods. 2015 Mar;12(3):207-10. doi: 10.1038/nmeth.3279. Epub
2015 Feb 2.
PUBMED 25643149
REFERENCE 2 (bases 1 to 12920)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Feb 27, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12920
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter complement(1721..1739)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 1752..1776
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 1912..1932
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 1933..3363
/codon_start=1
/product="tandem dimeric (pseudo-monomeric) derivative of
DsRed (Shaner et al., 2004)"
/label=tdTomato
/note="mammalian codon-optimized"
/translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
RSEGRHHLFLYGMDELYK"
promoter complement(5352..5370)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 5383..5407
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 5543..5563
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 5594..6310
/codon_start=1
/product="Venus YFP with monomerizing A206K mutation (Nagai
et al., 2002)"
/label=mVenus
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 6329..6349
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 6380..7096
/codon_start=1
/product="Venus YFP with monomerizing A206K mutation (Nagai
et al., 2002)"
/label=mVenus
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 7130..7849
/codon_start=1
/product="Venus YFP with monomerizing A206K mutation (Nagai
et al., 2002)"
/label=mVenus
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(8133..8153)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(8133..8151)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(8172..8188)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8172..8188)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(8185..8207)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 8196..8212
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8220..8250)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8265..8286
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8403..8420)
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 8525..8549
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
rep_origin complement(8640..9228)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8720..8739)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9399..10214)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind 10122..10141
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
primer_bind 10206..10225
/label=pENTR-R
/note="pENTR vectors, reverse primer"
rep_origin 10505..10940
/label=pSa ori
/note="origin of replication from bacterial plasmid pSa"
misc_feature 11067..11089
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA
(truncated)"
polyA_signal 11097..11273
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(11669..12232)
/codon_start=1
/gene="Mus musculus Dhfr"
/product="mouse dihydrofolate reductase"
/label=DHFR
/translation="MVRPLNCIVAVSQNMGIGKNGDRPWPPLRNEFKYFQRMTTTSSVE
GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
PGVLSEVQEEKGIKYKFEVYEKKD"
promoter complement(12265..12610)
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
primer_bind complement(12302..12322)
/label=35S promoter
/note="CaMV 35S promoter, forward primer"
primer_bind 12813..12835
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 12827..12844
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 12828..12844
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 12854..12872
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 tagttggaat aggatttccc gggccataat cgtgagtaga tatattactc aacttttgat
61 tcgctatttg cagtgcacct gtggcgttca tcacatcttt tgtggcactg tttgcactgg
121 tcattgctat tacaaaggac cttcctgatg ttgaaggaga tcgaaagtaa gtaactgcac
181 gcataaccat tttctttccg ctctttggct caatccattt gacagtcaaa gacaatgttt
241 aaccagctcc gtttgatata ttgtctttat gtgtttgttc aagcatgttt agttaatcat
301 gcctttgatt gatcttgaat aggttccaaa tatcaaccct ggcaacaaaa cttggagtga
361 gaaacattgc attcctcggt tctggacttc tgctagtaaa ttatgcttca gccatatcac
421 tagctttcta catgcctcag gtgaattcat ctatttccgt cttaactatt tcggttaatt
481 aaagcacgaa caccattact gcatgtagaa gcttgataaa ctatcgccac caatttattt
541 ttgttgcgat attgttactt tcctcagtat gcagctttga aaagaccaac cctcttatcc
601 tttaacaatg aacaggtttt tagaggtagc ttgatgattc ctgcacatgt gatcttggct
661 tcaggcttaa ttttccaggt aaagcattat gagatactct tatatctctt acatactttt
721 gagataatgc acaagaactt cataactata tgctttagtt tctgcatttg acactgccaa
781 attcattaat ctctaatatc tttgttgttg atctttggta gacatgggta ctagaaaaag
841 caaactacac caaggtaaaa tacttttgta caaacataaa ctcgttatca cggaacatca
901 atggagtgta tatctaacgg agtgtagaaa catttgatta ttgcaggaag ctatctcagg
961 atattatcgg tttatatgga atctcttcta cgcagagtat ctgttattcc ccttcctcta
1021 gctttcaatt tcatggtgag gatatgcagt tttctttgta tatcattctt cttcttcttt
1081 gtagcttgga gtcaaaatcg gttccttcat gtacatacat caaggatatg tccttctgaa
1141 tttttatatc ttgcaataaa aatgcttgta ccaattgaaa caccagcttt ttgagttcta
1201 tgatcactga cttggttcta accaaaaaaa aaaatgttta atttacatat ctaaaagtag
1261 gtttagggaa acctaaacag taaaatattt gtatattatt cgaatttcac tcatcataaa
1321 aacttaaatt gcaccataaa attttgtttt actattaatg atgtaatttg tgtaacttaa
1381 gataaaaata atattccgta agttaaccgg ctaaaaccac gtataaacca gggaacctgt
1441 taaaccggtt ctttactgga taaagaaatg aaagcccatg tagacagctc cattagagcc
1501 caaaccctaa atttctcatc tatataaaag gagtgacatt agggtttttg ttcgtcctct
1561 taaagcttct cgttttctct gccgtctctc tcattcgcgc gacgcaaacg atcttcaggt
1621 gatcttcttt ctccaaatcc tctctcataa ctctgatttc gtacttgtgt atttgagctc
1681 acgctctgtt tctctcacca cagccgggta cccaattcgc cctatagtga gtcgtattac
1741 aattcactgg cccaagtttg tacaaaaaag caggctatga aacaaaaaag ctcgaccaaa
1801 gaaacatcat tcctttccaa taacagggtt gaggtagctc cagtggtggg atggctgccg
1861 gtgagatcat cccggagagg gaaatccaac tcaatactga agcttatggc tccaaagaag
1921 aagagaaagg tcatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag
1981 gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc
2041 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
2101 ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac
2161 cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc
2221 gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac
2281 ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta
2341 atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc
2401 gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg
2461 gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg
2521 gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag
2581 cgctccgagg gccgccacca cctgttcctg gggcatggca ccggcagcac cggcagcggc
2641 agctccggca ccgcctcctc cgaggacaac aacatggccg tcatcaaaga gttcatgcgc
2701 ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc
2761 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc
2821 ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg
2881 aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg
2941 gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg
3001 caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc
3061 cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc
3121 gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac
3181 ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac
3241 tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag
3301 tacgagcgct ccgagggccg ccaccacctg ttcctgtacg gcatggacga gctgtacaag
3361 tagctcgaga gatccgatcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt
3421 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
3481 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
3541 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
3601 attatcgcgc gcggtgtcat ctatgtgaat tctagttgga atagggttcg ggccataatc
3661 gtgagtagat atattactca acttttgatt cgctatttgc agtgcacctg tggcgttcat
3721 cacatctttt gtggcactgt ttgcactggt cattgctatt acaaaggacc ttcctgatgt
3781 tgaaggagat cgaaagtaag taactgcacg cataaccatt ttctttccgc tctttggctc
3841 aatccatttg acagtcaaag acaatgttta accagctccg tttgatatat tgtctttatg
3901 tgtttgttca agcatgttta gttaatcatg cctttgattg atcttgaata ggttccaaat
3961 atcaaccctg gcaacaaaac ttggagtgag aaacattgca ttcctcggtt ctggacttct
4021 gctagtaaat tatgcttcag ccatatcact agctttctac atgcctcagg tgaattcatc
4081 tatttccgtc ttaactattt cggttaatta aagcacgaac accattactg catgtagaag
4141 cttgataaac tatcgccacc aatttatttt tgttgcgata ttgttacttt cctcagtatg
4201 cagctttgaa aagaccaacc ctcttatcct ttaacaatga acaggttttt agaggtagct
4261 tgatgattcc tgcacatgtg atcttggctt caggcttaat tttccaggta aagcattatg
4321 agatactctt atatctctta catacttttg agataatgca caagaacttc ataactatat
4381 gctttagttt ctgcatttga cactgccaaa ttcattaatc tctaatatct ttgttgttga
4441 tctttggtag acatgggtac tagaaaaagc aaactacacc aaggtaaaat acttttgtac
4501 aaacataaac tcgttatcac ggaacatcaa tggagtgtat atctaacgga gtgtagaaac
4561 atttgattat tgcaggaagc tatctcagga tattatcggt ttatatggaa tctcttctac
4621 gcagagtatc tgttattccc cttcctctag ctttcaattt catggtgagg atatgcagtt
4681 ttctttgtat atcattcttc ttcttctttg tagcttggag tcaaaatcgg ttccttcatg
4741 tacatacatc aaggatatgt ccttctgaat ttttatatct tgcaataaaa atgcttgtac
4801 caattgaaac accagctttt tgagttctat gatcactgac ttggttctaa ccaaaaaaaa
4861 aaaaatgttt aatttacata tctaaaagta ggtttaggga aacctaaaca gtaaaatatt
4921 tgtatattat tcgaatttca ctcatcataa aaacttaaat tgcaccataa aattttgttt
4981 tactattaat gatgtaattt gtgtaactta agataaaaat aatattccgt aagttaaccg
5041 gctaaaacca cgtataaacc agggaacctg ttaaaccggt tctttactgg ataaagaaat
5101 gaaagcccat gtagacagct ccattagagc ccaaacccta aatttctcat ctatataaaa
5161 ggagtgacat tagggttttt gttcgtcctc ttaaagcttc tcgttttctc tgccgtctct
5221 ctcattcgcg cgacgcaaac gatcttcagg tgatcttctt tctccaaatc ctctctcata
5281 actctgattt cgtacttgtg tatttgagct cacgctctgt ttctctcacc acagccgggt
5341 acccaattcg ccctatagtg agtcgtatta caattcactg gcccaagttt gtacaaaaaa
5401 gcaggctatg aaacaaaaaa gctcgaccaa agaaacatca ttcctttcca ataacagggt
5461 tgaggtagct ccagtggtgg gatggccgcc ggtgagatca tcccggagag aacccaactc
5521 catactggat ccaacaatgg ctcccaagaa gaagagaaag gtagatcccc gggctgcagg
5581 aattaattcg atcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
5641 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
5701 cgatgccacc tacggcaagc tgaccctgaa gctgatctgc accaccggca agctgcccgt
5761 gccctggccc accctcgtga ccaccctggg ctacggcctg cagtgcttcg cccgctaccc
5821 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
5881 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
5941 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
6001 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcaccgccga
6061 caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcgg
6121 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
6181 gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg
6241 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
6301 gctgtacaag ttagatccaa caatggctcc caagaagaag agaaaggtag atccccgggc
6361 tgcaggaatt aattcgatca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
6421 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
6481 cgagggcgat gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct
6541 gcccgtgccc tggcccaccc tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg
6601 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
6661 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
6721 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
6781 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcac
6841 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga
6901 cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
6961 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga
7021 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
7081 ggacgagctg tacaagttag atccaggagc aggagcagga gcaggagcca tggtgagcaa
7141 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
7201 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
7261 cctgaagctg atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
7321 cctgggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
7381 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
7441 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
7501 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
7561 caactacaac agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc
7621 caacttcaag atccgccaca acatcgagga cggcggcgtg cagctcgctg accactacca
7681 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta
7741 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
7801 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag atcctctaga
7861 gtccgcaaaa atcaccagtc tctctctaca aatctatctc tctctatttt tctccagaat
7921 aatgtgtgag tagttcccag ataagggaat tagggttctt atagggtttc gctcatgtgt
7981 tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa
8041 tttctaattc ctaaaaccaa aatccagtga cctgcaggca tgcaagctta tctagagcgg
8101 ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg
8161 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
8221 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
8281 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
8341 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
8401 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
8461 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgaag
8521 gccttgacag gatatattgg cgggtaaact aagtcgctgt atgtgtttgt ttgagatctc
8581 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
8641 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
8701 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
8761 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
8821 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
8881 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
8941 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
9001 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
9061 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
9121 ttcggaagaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
9181 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
9241 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
9301 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
9361 tcaatctaaa gtatatatgt gtaacattgg tctagtgatt agaaaaactc atcgagcatc
9421 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
9481 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
9541 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
9601 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
9661 agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
9721 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
9781 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
9841 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
9901 gttttccctg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
9961 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
10021 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
10081 ccatacaatc ggtagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
10141 ccatataaat cagcatccat gttggaattt aatcgcggcc ttgagcaaga cgtttcccgt
10201 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
10261 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg
10321 ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agatcacgca tcttcccgac
10381 aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc cacctacaac
10441 aaagctctca tcaaccgtgg ctccctcact ttctggctgg atgatggggc gattcaggcg
10501 atccccatcc aacagcccgc cgtcgagcgg gcttttttat ccccggaagc ctgtggatag
10561 agggtagtta tccacgtgaa accgctaatg ccccgcaaag ccttgattca cggggctttc
10621 cggcccgctc caaaaactat ccacgtgaaa tcgctaatca gggtacgtga aatcgctaat
10681 cggagtacgt gaaatcgcta ataaggtcac gtgaaatcgc taatcaaaaa ggcacgtgag
10741 aacgctaata gccctttcag atcaacagct tgcaaacacc cctcgctccg gcaagtagtt
10801 acagcaagta gtatgttcaa ttagcttttc aattatgaat atatatatca attattggtc
10861 gcccttggct tgtggacaat gcgctacgcg caccggctcc gcccgtggac aaccgcaagc
10921 ggttgcccac cgtcgagcgc ctttgcccac aacccggcgg ccggccgcaa cagatcgttt
10981 tataaatttt tttttttgaa aaagaaaaag cccgaaaggc ggcaacctct cgggcttctg
11041 gatttccgat ccccggaatt agatcttggc aggatatatt gtggtgtaac gttatcgatc
11101 tggattttag tactggattt tggttttagg aattagaaat tttattgata gaagtatttt
11161 acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata
11221 agaaccctaa tttcccttat cgggaaacta ctcacacatt atttatggag aaaatagaga
11281 gagatagatt tgtagagaga gactggtgat ttcagcgtac cgaattaatt cgagctcggt
11341 acccctggat tttggtttta ggaattagaa attttattga tagaagtatt ttacaaatac
11401 aaatacatac taagggtttc ttatatgctc aacacatgag cgaaacccta taagaaccct
11461 aattccctta tctgggaact actcacacat tattatagag agagatagat ttgtagagag
11521 agactggtga tttcagcggg ggattcagcg ccgacgggac gtaaacaaag gacgtcccgc
11581 gaaggatcta aagccagcaa aagtcccatg gtcttataaa aatgcatagc tttaggaggg
11641 gagcagagaa cttgaaagca tcttcctgtt agtctttctt ctcgtagact tcaaacttat
11701 acttgatgcc tttttcctcc tggacctcag agaggacgcc tgggtattct gggagaagtt
11761 tatatttccc caaatcaatt tctgggaaaa acgtgtcact ttcaaattcc tgcatgatcc
11821 ttgtcacaaa gagtctgagg tggcctggtt gattcatggc ttcctggtaa acagaactgc
11881 ctccgactat ccaaaccatg tctactttac ttgccaattc cggttgttca ataagtctta
11941 aggcatcatc caaacttttg gcaagaaaat gagctcctcg tggtggttct ttgagttctc
12001 tactgagaac tatattaatt ctgtccttta aaggtcgatt cttctcagga atggagaacc
12061 aggttttcct acccataatc accagattct gtttaccttc cactgaagag gttgtggtca
12121 ttctttggaa gtacttgaac tcgttcctga gcggaggcca gggtcggtct ccgttcttgc
12181 caatccccat attttgggac acggcgacga tgcagttcaa tggtcgaacc atgatggcaa
12241 attctagggg tacccagctt gggctgtcct ctccaaatga aatgaacttc cttatataga
12301 ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatgtc
12361 acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct
12421 cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa tgatagcctt
12481 tcctttatcg caatgatggc atttgtagga gccaccttcc ttttctactg tcctttcgat
12541 gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgaaatta ccctttgttg
12601 aaaagtctca atagcccttt ggtcttctga gactgtatct ttgacatttt tggagtaggg
12661 gtacgataac attaacgctt acaatttcca ttcgccattc aggctgcgca actgttggga
12721 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
12781 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
12841 cagtgagcgc gcgtaatacg actcactata gggcgaattg ggtaccgggc ccgtatggtt
12901 aattaagaca caggcgcgcc
//
